| 1. James Carter & The Prisoners, Charles Mitchell - Po' Lazarus | not rated | 4:31 |
| 2. Harry McClintock, James Carter and the Prisoners - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake, Harry McClintock - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss, Traditional - Down to the River to Pray | not rated | 2:55 |
| 5. Soggy Bottom Boys, The, Dan Tyminski, Traditional - I Am A Man Of Constant Sorrow | not rated | 3:10 |
| 6. Chris Thomas King, Traditional - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. Norman Blake, Gary Garett - I Am A Man Of Constant Sorrow (Instrumental) | not rated | 4:29 |
| 8. Whites, The, Skip James - Keep On The Sunny Side | not rated | 3:34 |
| 9. Gillian Welch, Alison Krauss, Albert E. Brumley - I'll Fly Away | not rated | 3:57 |
| 10. Gillian Welch, Alison Krauss, Emmylou Harris, Gillian Welch - Didn't Leave Nobody But the Baby | not rated | 1:57 |
| 11. Sarah, Hanna and Leah Peasall, Traditional - In the Highways | not rated | 1:36 |
| 12. Cox Family, The, Mother Maybelle Carter - I Am Weary (Let Me Rest) | not rated | 3:14 |
| 13. John Hartford, Traditional - I Am A Man Of Constant Sorrow (Instrumental) | not rated | 2:34 |
| 14. Ralph Stanley, Traditional - O Death | not rated | 3:19 |
| 15. Soggy Bottom Boys, The - In The Jailhouse Now | not rated | 3:36 |
| 16. Soggy Bottom Boys, The, Hoyt "Floyd" Ming - I Am A Man Of Constant Sorrow | not rated | 4:16 |
| 17. John Hartford, Jimmie Rodgers - Indian War Whoop | not rated | 1:30 |
| 18. Fairfield Four, The, Traditional - Lonesome Valley | not rated | 4:07 |
| 19. Stanley Brothers, The, Traditional - Angel Band | not rated | 2:17 |
| 1:00:37 |
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