| 1. Terry, Sonny (Sonny Terry) and Brownie McGhee - Old Jabo | not rated | 2:14 |
| 2. Broonzy, Big Bill (Big Bill Broonzy) - Mule Ridin' Blues | not rated | 3:46 |
| 3. Memphos Slim and Willie Dixon - Joogie Boogie | not rated | 3:27 |
| 4. Hall, Vera (Vera Hall) - Black Woman | not rated | 1:27 |
| 5. Douglas, K.C. (K.C. Douglas) - Mercury Blues | not rated | 2:21 |
| 6. Sykes, Roosevelt (Roosevelt Sykes) - Ran the Blues Out of My Window | not rated | 2:57 |
| 7. Leadbelly - Leaving Blues | not rated | 2:23 |
| 8. Baker, Etta (Etta Baker) - One Dime Blues | not rated | 3:46 |
| 9. House, Son (Son House) - County Farm Blues | not rated | 2:11 |
| 10. Dupree, Champion Jack (Champion Jack Dupree) - Clog Dance (Stomping Blues) | not rated | 2:02 |
| 11. Anderson, Pink (Pink Anderson) - Boll Weevil | not rated | 3:06 |
| 12. Johnson, Edith North (Edith North Johnson) & Henry Brown - Nickel's Worth of Liver | not rated | 2:43 |
| 13. Williams, Big Joe (Big Joe Williams) - Don't Leave Me Here | not rated | 2:43 |
| 14. Iron, Cat (Cat Iron) - Jimmy Bell | not rated | 2:20 |
| 15. Davis, Rev. Gary (Rev. Gary Davis) - Candy Man | not rated | 2:35 |
| 16. Memphis Slim & Willie Dixon - Beer Drinking Woman | not rated | 2:32 |
| 17. Hopkins, Lightnin' (Lightnin' Hopkins) - Come Go Home with Me | not rated | 3:55 |
| 18. White, Josh (Josh White) - Careless Love | not rated | 3:23 |
| 19. Townsend, Henry (Henry Townsend) - I Asked Her If She Loved Me | not rated | 2:55 |
| 20. McGhee, Brownie (Brownie McGhee) and Sonny Terry - Rising Sun | not rated | 2:43 |
| 21. Edwards, Honeyboy (Honeyboy Edwards) - Pony Blues | not rated | 3:28 |
| 22. Montgomery, Little Brother (Little Brother Montgomery) - Vicksburg Blues | not rated | 4:02 |
| 23. Cotten, Elizabeth (Elizabeth Cotten) - Vastapol | not rated | 2:11 |
| 24. Johnson, Lonnie (Lonnie Johnson) - Drifting Along Blues | not rated | 2:59 |
| 25. The Chambers Brothers - Oh Baby, You Don't Have to Go | not rated | 2:49 |
| 26. Lead Belly & Josh White - Don't Lie Buddy | not rated | 2:20 |
| 1:13:31 |
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