| 1. James Carter & the Prisoners, James Carter And The Prisoners - Po' Lazarus | not rated | 4:30 |
| 2. Harry McClintock, Harry McClintock - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake, Charles Mitchell/Jimmie Davis - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss, Traditional - Down to the River to Pray | not rated | 2:55 |
| 5. The Soggy Bottom Boys, Traditional - I am a Man of Constant Sorrow | not rated | 3:09 |
| 6. Chris Thomas King, Skip James - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. Norman Blake, Traditional - I am a Man of Constant Sorrow [Instrumental] | not rated | 4:29 |
| 8. The Whites, A.P. Carter/Gary Garett - Keep on the Sunny Side | not rated | 3:34 |
| 9. Alison Krauss and Gillian Welch, Albert E. Brumley - I'll Fly Away | not rated | 3:57 |
| 10. Emmylou Harris, Alison Krauss and Gillian Welch, Gillian Welch/T-Bone Burnett - Didn't Leave Nobody But the Baby | not rated | 1:57 |
| 11. Sarah, Hannah and Leah Peasall, Mother Maybelle Carter - In the Highways | not rated | 1:36 |
| 12. The Cox Family, Tete Roberts - I Am Weary (Let Me Rest) | not rated | 3:14 |
| 13. John Hartford, Traditional - I am a Man of Constant Sorrow [Instrumental] | not rated | 2:34 |
| 14. Ralph Stanley, Traditional - O Death | not rated | 3:19 |
| 15. The Soggy Bottom Boys, Jimmie Rodgers - In the Jailhouse Now | not rated | 3:36 |
| 16. The Soggy Bottom Boys, Traditional - I am a Man of Constant Sorrow [Band Version] | not rated | 4:16 |
| 17. John Hartford, Hoyt "Floyd" Ming - Indian War Whoop [Instrumental] | not rated | 1:30 |
| 18. Fairfield Four, Traditional - Lonesome Valley | not rated | 4:07 |
| 19. The Stanley Brothers, Traditional/William York - Angel Band | not rated | 2:15 |
| 1:00:24 |
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