
| 1. James Carter & the Prisoners - Po' Lazarus | not rated | 4:31 |
| 2. Harry McClintock - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss - Down to the River to Pray | not rated | 2:55 |
| 5. The Soggy Bottom Boys - I Am a Man of Constant Sorrow (radio station version) | not rated | 3:10 |
| 6. Chris Thomas King - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. Norman Blake - I Am a Man of Constant Sorrow (instrumental) | not rated | 4:29 |
| 8. The Whites - Keep on the Sunny Side | not rated | 3:34 |
| 9. Gillian Welch & Alison Krauss - I'll Fly Away | not rated | 3:57 |
| 10. Gillian Welch, Alison Krauss & Emmylou Harris - Didn't Leave Nobody but the Baby | not rated | 1:57 |
| 11. The Peasall Sisters - In the Highways | not rated | 1:36 |
| 12. The Cox Family - I Am Weary (Let Me Rest) | not rated | 3:14 |
| 13. John Hartford - I Am a Man of Constant Sorrow (instrumental) | not rated | 2:34 |
| 14. Ralph Stanley - O Death | not rated | 3:19 |
| 15. The Soggy Bottom Boys - In the Jailhouse Now | not rated | 3:36 |
| 16. The Soggy Bottom Boys - I Am a Man of Constant Sorrow (with band) | not rated | 4:16 |
| 17. John Hartford - Indian War Whoop | not rated | 1:30 |
| 18. The Fairfield Four - Lonesome Valley | not rated | 4:07 |
| 19. The Stanley Brothers - Angel Band | not rated | 2:15 |
| 1:00:35 | ||
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