| 1. James Carter And The Prisoners, James Carter And The Prisoners - Po' Lazarus | not rated | 4:31 |
| 2. Harry McClintock, Harry McClintock - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake, Charles Mitchell/Jimmie Davis - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss, Traditional - Down to the River to Pray | not rated | 2:56 |
| 5. Soggy Bottom Boys, Dan Tyminski, Traditional - Man of Constant Sorrow | not rated | 3:10 |
| 6. Chris Thomas King, Skip James - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. Norman Blake, Traditional - Man of Constant Sorrow [Instrumental] | not rated | 4:29 |
| 8. The Whites, A.P. Carter/Gary Garett - Keep on the Sunny Side | not rated | 3:34 |
| 9. Alison Krauss, Gillian Welch, Albert E. Brumley - I'll Fly Away | not rated | 3:58 |
| 10. Emmylou Harris, Alison Krauss, Gillian Welch, Gillian Welch/T-Bone Burnett - Didn't Leave Nobody But the Baby | not rated | 1:58 |
| 11. The Peasall Sisters, Hannah Peasall, Leah Peasall, Sarah Peasall, Mother Maybelle Carter - In the Highways | not rated | 1:36 |
| 12. The Cox Family, Tete Roberts - I Am Weary, Let Me Rest | not rated | 3:14 |
| 13. John Hartford, Traditional - Man of Constant Sorrow [Instrumental] | not rated | 2:34 |
| 14. Ralph Stanley, Traditional - O Death | not rated | 3:19 |
| 15. Tim Blake Nelson, Soggy Bottom Boys, Jimmie Rodgers - In the Jailhouse Now | not rated | 3:36 |
| 16. Soggy Bottom Boys, Dan Tyminski, Traditional - Man of Constant Sorrow [Band Version][Take] | not rated | 4:17 |
| 17. John Hartford, Hoyt "Floyd" Ming - Indian War Whoop [Instrumental] | not rated | 1:30 |
| 18. The Fairfield Four, Traditional - Lonesome Valley | not rated | 4:07 |
| 19. The Stanley Brothers, Traditional/William York - Angel Band | not rated | 2:17 |
| 1:00:32 |
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