| 1. Flatt & Scruggs - Froggy Mountain Breakdown | not rated | 2:40 |
| 2. Bill Monroe & His Bluegrass Boys - Blue Moon of Kentucky | not rated | 2:01 |
| 3. Stanley Brothers, The - I'm a Man of Constant Sorrow | not rated | 2:57 |
| 4. Johnny Duncan & The Blue Grass Boys - Last Train to San Fernando | not rated | 2:28 |
| 5. Reno & Smiley - I'm Gone, Long Gone | not rated | 2:19 |
| 6. Cumberland Mountain Folks, The, Molly O'Day - Poor Ellen Smith | not rated | 2:40 |
| 7. Jimmy Martin, Osborne Brothers - She's Just a Cute Thing | not rated | 1:56 |
| 8. Clinch Mountain Boys, Stoney Cooper, Wilma Lee Cooper - Are You Walking and A-Talking for the Lord | not rated | 2:06 |
| 9. Mac Wiseman - Going Like Wildfire | not rated | 2:31 |
| 10. Lonesome Pine Fiddlers - Pain in My Heart | not rated | 2:43 |
| 11. Jim Eanes & His Shenandoah Valley Boys - I Cried Again | not rated | 3:07 |
| 12. Frank Hunter & His Black Mountain Boys - Tenessee Boy | not rated | 3:17 |
| 13. Sonny Osborne - I'm Gonna Leave You | not rated | 2:40 |
| 14. Bill Monroe & His Bluegrass Boys - Y'all Come | not rated | 2:11 |
| 15. Flatt & Scruggs - Get in Line Brother | not rated | 2:17 |
| 16. Happy Valley Boys, Bailey Brothers, The - Beautiful Brown Eyes | not rated | 2:44 |
| 17. Stanley Brothers, The - Too Late to Cry | not rated | 2:59 |
| 18. Carl Story & the Rambling Mountaineers, Carl Story - He Will Set Your Fields on Fire | not rated | 2:23 |
| 19. Molly O'Day & The Cumberland Mountain Folks - Higher in My Prayer | not rated | 2:28 |
| 20. Bailes Brothers, The - Something Got Hold of Me | not rated | 2:45 |
| 21. Reno & Smiley - Tennessee Cut-Up Breakdown | not rated | 1:41 |
| 22. Blue Sky Boys, The - Garden in the Sky | not rated | 2:46 |
| 23. Mac Wiseman - Dreaming of a Little Cabin | not rated | 2:54 |
| 24. Virginia Boys, Jim & Jesse - Are You Missing Me? | not rated | 2:24 |
| 25. Brown's Ferry Four - Will the Circle Be Unbroken? | not rated | 2:57 |
| 1:04:05 |
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