| 1. Bill Monroe & His Bluegrass Boys - Orange Blossom Special | not rated | 2:33 |
| 2. Stanley Brothers, The - Cotton-Eyed Joe | not rated | 1:13 |
| 3. Earl Scruggs, Flatt & Scruggs - Roll in My Sweet Baby's Arms | not rated | 2:38 |
| 4. Tommy Magness & The Tennessee Buddies - When I Safely Reach That Other Shore | not rated | 2:33 |
| 5. Wilma Lee & Stoney Cooper - Sunny Side of the Mountain | not rated | 3:04 |
| 6. Jim Eanes & the Shenandoah Valley Boys - Tomorrow May Be Different | not rated | 2:38 |
| 7. Lonesome Pine Fiddlers - Lonesome Pine Breakdown | not rated | 2:30 |
| 8. Ralph Mayo, L.C. Smith, Southern Mountain Boys - Radio Boogie | not rated | 2:25 |
| 9. Reno & Smiley - Crazy Finger Blues | not rated | 2:44 |
| 10. Molly O'Day & The Cumberland Mountain Folks - The Tramp on the Street | not rated | 2:48 |
| 11. Earl Scruggs, Flatt & Scruggs - Don't Get Above Your Raisin' | not rated | 2:43 |
| 12. Stanley Brothers, The - The Fields Have Turned Brown | not rated | 2:33 |
| 13. Bill Monroe & His Bluegrass Boys - Molly and Tenbrooks (The Race Horse Song) | not rated | 2:46 |
| 14. Hobo Jack Adkins - Going Back to Old Kentucky | not rated | 2:05 |
| 15. Lonesome Pine Fiddlers - Twenty One Years | not rated | 2:20 |
| 16. Mac Wiseman - Tis Sweet to Be Remembered | not rated | 2:56 |
| 17. Jim Eanes & the Shenandoah Valley Boys - Florida Blues | not rated | 2:21 |
| 18. Wade Mainer - Little Birdie | not rated | 2:48 |
| 19. Shannon Grayson - I Like the Old Time Way | not rated | 2:57 |
| 20. Flatt & Scruggs - Doin' My Time | not rated | 2:48 |
| 21. Stanley Brothers, The - This Little Glass of Wine | not rated | 3:05 |
| 22. Bailey Brothers, The - Happy Valley Special | not rated | 2:21 |
| 23. Lonesome Pine Fiddlers - Lonesome, Sad and Blue | not rated | 2:24 |
| 24. Frank Hunter & His Black Mountain Boys - Long Time, No See | not rated | 2:29 |
| 25. Jim Eanes & the Shenandoah Valley Boys, Jim Eanes - Blue Yodel No. 1 (T for Texas) | not rated | 2:18 |
| 1:04:12 |
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