| Disc 1 |
| 1. Othar Turner/traditional - Shortin' / Hunduck | not rated | 2:52 |
| 2. traditional - Long John | not rated | 5:26 |
| 3. Bradford / traditional - Crazy Blues | not rated | 3:29 |
| 4. W.C. Handy - St. Louis Blues | not rated | 3:02 |
| 5. DeRose / Richman / Trent - Muddy Water | not rated | 3:08 |
| 6. Match Box Blues | not rated | 3:02 |
| 7. Billy Lyons & Stack-O-Lee | not rated | 2:36 |
| 8. Gertrude Rainey - Ma Rainey's Black Bottom | not rated | 3:03 |
| 9. Blind Willie Johnson - Dark was the Night, Cold was the Ground | not rated | 3:21 |
| 10. E. Ory - Savoy Blues | not rated | 3:28 |
| 11. Dan Sane / Frank Stokes - Downtown Blues | not rated | 3:10 |
| 12. Mississippi John Hurt - Frankie | not rated | 3:24 |
| 13. Henry Thomas - Fishing Blues | not rated | 2:43 |
| 14. Leroy Carr - How Long, How Long Blues | not rated | 3:06 |
| 15. Tommy Johnson - Canned Heat Blues | not rated | 3:38 |
| 16. William McTell - Statesboro Blues | not rated | 2:30 |
| 17. Thomas A. Dorsey / Hudson Whitaker - It's Tight Like That | not rated | 3:05 |
| 18. Clarence Smith - Pine Top's Boogie Woogie | not rated | 3:22 |
| 19. Lonnie Johnson / Eddie Lang - Guitar Blues | not rated | 3:16 |
| 20. Charley Patton - Pony Blues | not rated | 3:00 |
| 21. Arthur Phelps - Diddie Wah Diddie | not rated | 2:58 |
| 22. Tewee Blackman - K.C. Moan | not rated | 2:32 |
| 23. Jimmie Rodgers - Standin' on the Corner (Blue Yodel #9) | not rated | 2:39 |
| 24. Walter Vinson - Sittin' on Top of the World | not rated | 3:12 |
| 25. Eddie James ''Son'' House, Jr. - Preachin' the Blues | not rated | 3:02 |
| 1:19:04 |
| Disc 2 |
| 1. Nehemiah James - Devil Got My Woman | not rated | 3:01 |
| 2. Huddie Ledbetter / traditional - C.C. Rider | not rated | 2:59 |
| 3. Big Joe Williams - Baby, Please Don't Go | not rated | 3:24 |
| 4. Roosevelt Sykes - Dirty Mother for You (Don't You Know?) | not rated | 3:00 |
| 5. Billie Holiday - Billie's Blues | not rated | 2:40 |
| 6. Robert Johnson - Cross Road Blues | not rated | 2:40 |
| 7. John Lee Williamson - Good Mornin', Little School Girl | not rated | 3:01 |
| 8. Booker T. Washington White / traditional - Shake 'em on Down | not rated | 3:02 |
| 9. Joe Turner / Pete Johnson - Roll 'em, Pete | not rated | 2:50 |
| 10. Robert Petway - Catfish Blues | not rated | 2:54 |
| 11. William Basie / Jimmy Rushing - Going to Chicago Blues | not rated | 3:26 |
| 12. Bill Broonzy / Segar - Key to the Highway | not rated | 3:01 |
| 13. Lizzie Douglas - Me and My Chauffeur Blues | not rated | 2:47 |
| 14. Maceo Merriweather - Worried Life Blues | not rated | 2:56 |
| 15. Tommy McClennon - Cross Cut Saw Blues | not rated | 2:46 |
| 16. Leonard Feather - Evil Gal Blues | not rated | 2:54 |
| 17. traditional / Sisiter Rosetta Tharpe - Strange Things Happening Everyday | not rated | 2:53 |
| 18. Joe Liggins - Honeydripper, part 1 | not rated | 3:05 |
| 19. Charles Brown / Johnny Moore / Eddie Williams - Drifting Blues | not rated | 3:14 |
| 20. Sam Theard / Fleecie Moore - Let The Good Times Roll | not rated | 2:48 |
| 21. Arthur Crudup - That's All Right, Mama | not rated | 2:55 |
| 22. Aaron Thibideaux Walker - Call It Stormy Monday | not rated | 3:02 |
| 23. Roy Brown - Good Rockin' Tonight | not rated | 2:48 |
| 24. P. Granger / Jimmy Witherspoon / R. Prince / C. Williams - Ain't Nobody's Business, part 1 | not rated | 2:55 |
| 25. Johnny Otis - Double Crossing Blues | not rated | 2:47 |
| 1:13:48 |
| Disc 3 |
| 1. John Peter Chatman / Lewis Simkins - Mother Earth | not rated | 2:42 |
| 2. Percy Mayfield - Please Send Me Someone to Love | not rated | 2:55 |
| 3. Ike Turner - Rocket '88 | not rated | 2:50 |
| 4. Robert Johnson - (I Believe I'll) Dust My Broom | not rated | 2:45 |
| 5. Rosco Gordon - No More Doggin' | not rated | 3:09 |
| 6. Walter Jacobs - Juke | not rated | 2:46 |
| 7. Jerry Lieber / Mike Stoller - Hound Dog | not rated | 2:50 |
| 8. Lowell Fulson - Reconsider, Baby | not rated | 3:10 |
| 9. Eddie James ''Son'' House Jr - The Things I Used to Do | not rated | 3:02 |
| 10. Roy Byrd - In the Night | not rated | 2:32 |
| 11. Willie Dixon - (I'm Your) Hoochie Coochie Man | not rated | 2:51 |
| 12. J.B. Lenoir - Eisenhower Blues | not rated | 2:54 |
| 13. Antoine Domino / Dave Bartholomew - Blue Monday | not rated | 2:17 |
| 14. Ray Charles - Hard Times | not rated | 2:55 |
| 15. Dave Bartholomew / Pearl King - I Hear You Knockin' | not rated | 2:44 |
| 16. Junior Parker / Sam Phillips - Mystery Train | not rated | 2:27 |
| 17. Aleck Rice Miller - Don't Start Me to Talkin' | not rated | 2:34 |
| 18. Chester Arthur Burnett - Smokestack Lightin' | not rated | 3:08 |
| 19. Elias McDaniel - Who Do You Love? | not rated | 2:30 |
| 20. James Moore - I'm a King Bee | not rated | 3:05 |
| 21. Chuck Berry - Johnny B. Goode | not rated | 2:42 |
| 22. Don Robey / Joe Veasy - Farther Up The Road | not rated | 2:59 |
| 23. Marshall Paul - So Many Roads, So Many Trains | not rated | 3:13 |
| 24. Eurreal Montgomery - First Time I Met the Blues | not rated | 2:18 |
| 25. Willie Dixon - Big Boss Man | not rated | 2:49 |
| 1:10:07 |
| Disc 4 |
| 1. Freddie King - Hide Away | not rated | 2:36 |
| 2. Roosevelt Sykes - Drivin' Wheel | not rated | 2:37 |
| 3. John Lee Hooker - Boom Boom | not rated | 2:32 |
| 4. Albert Collins - Frosty | not rated | 3:04 |
| 5. McKinley Morganfield - You Can't Lose What You Ain't Never Had | not rated | 2:58 |
| 6. Chester Arthur Burnett - Killing Floor | not rated | 2:50 |
| 7. Eddie James ''Son'' House, Jr. - Death Letter Blues | not rated | 4:21 |
| 8. Fred McDowell - You Gotta Move | not rated | 3:22 |
| 9. Bob Dylan - Highway 61 Revisted | not rated | 3:26 |
| 10. Junior Wells - Hoodoo Man Blues | not rated | 2:05 |
| 11. Willie Dixon - Wang Dang Doodle | not rated | 3:01 |
| 12. All Your Love | not rated | 3:34 |
| 13. traditional - I've Got a Mind to Give Up Livin' | not rated | 4:58 |
| 14. Jimi Hendrix - Red House | not rated | 3:51 |
| 15. Booker T. Jones / William Bell - Born under a Bad Sign | not rated | 2:46 |
| 16. J.B. Lenoir - Mama, Talk to Your Daughter | not rated | 2:43 |
| 17. Clarence Carter / Wilbur Terrell / Marcus Daniels - Tell Mama | not rated | 2:23 |
| 18. Willie Dixon - Ain't Superstitious | not rated | 4:54 |
| 19. Taj Mahal / James Rachel - She Caught the Katy (and Left Me a Mule to Ride) | not rated | 3:30 |
| 20. Peter Green - Black Magic Woman | not rated | 2:53 |
| 21. Janis Joplin - One Good Man | not rated | 4:09 |
| 1:08:33 |
| Disc 5 |
| 1. Rick Darnell / Roy Hawkins - The Thrill is Gone | not rated | 4:03 |
| 2. Johnny Winter - Dallas | not rated | 2:45 |
| 3. Billy Myles - Have You Ever Loved a Woman? | not rated | 6:54 |
| 4. Theodore Taylor - Give Me Back My Wig | not rated | 3:36 |
| 5. Marshall Sehorn / Elmore James - One Way Out | not rated | 4:57 |
| 6. George Jackson - Down Home Blues | not rated | 3:59 |
| 7. Stevie Ray Vaughan - Pride and Joy | not rated | 3:40 |
| 8. Robert Cray / Richard Cousins / Bruce Bromberg - Smoking Gun | not rated | 4:01 |
| 9. Kim Wilson - Tuff Enuff | not rated | 3:22 |
| 10. John Lee Hooker / Bernard Bessman - I'm in the Mood | not rated | 4:31 |
| 11. Ali Farka Touré - Timbarma | not rated | 4:11 |
| 12. Kevin Moore - Am I Wrong? | not rated | 2:20 |
| 13. Luther Allison - Cherry Red Wine | not rated | 4:23 |
| 14. Susan Tedeschi - Just Won't Burn | not rated | 4:46 |
| 15. J.B. Lenoir - Voodoo Music | not rated | 3:23 |
| 16. J.B. Lenoir / Willie Dixon - Round & Round | not rated | 3:39 |
| 17. J.B. Lenoir - Vietnam Blues | not rated | 4:32 |
| 18. Deadric Malone - I Pity the Fool (live) | not rated | 3:40 |
| 19. Robert Johnson - Sweet Home Chicago | not rated | 2:51 |
| 20. Jimmy Lewis - Bill | not rated | 4:19 |
| 1:19:52 |
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