| 1. James Carter & The Prisoners, Traditional, arr. by Alan Lomax - Po' Lazarus | not rated | 4:30 |
| 2. Harry McClintock, Harry McClintock - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake, Jimmie Davis, Charles Mitchell - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss, Traditional, arr. by Allison Krauss - Down To The River To Pray | not rated | 2:56 |
| 5. The Soggy Bottom Boys, Traditional, arr. by Carter Stanley - I Am A Man Of Constant Sorrow | not rated | 3:10 |
| 6. Chris Thomas King, Nehemiah "Skip" James - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. John Hartford, Traditional, arr. by Norman Blake - I Am A Man Of Constant Sorrow (Instrumental) | not rated | 4:29 |
| 8. The Whites, A.P. Carter - Keep On The Sunny Side | not rated | 3:34 |
| 9. Alison Krauss & Gillian Welch, Albert E. Brumley - I'll Fly Away | not rated | 3:57 |
| 10. Emmylou Harris, Alison Krauss & Gillian Welch, Traditional, arr. by Alan Lomax, Mrs. Sidney Carter, Gillian Welch, and T Bone B - Didn't Leave Nobody But The Baby | not rated | 1:57 |
| 11. The Peasall Sisters, Maybelle Carter - In the Highways | not rated | 1:36 |
| 12. The Cox Family, Pete Roberts - I Am Weary (Let Me Rest) | not rated | 3:14 |
| 13. John Hartford, Traditional, arr. by Ed Haley - I Am A Man Of Constant Sorrow (Instrumental) | not rated | 2:34 |
| 14. Ralph Stanley, Traditional, arr. by Ralph Stanley - O Death | not rated | 3:19 |
| 15. The Soggy Bottom Boys, Jimmie Rodgers - In The Jailhouse Now | not rated | 3:36 |
| 16. The Soggy Bottom Boys, Traditional, arr. by Carter Stanley - I Am A Man Of Constant Sorrow | not rated | 4:17 |
| 17. John Hartford, Hoyt Ming - Indian War Whoop | not rated | 1:30 |
| 18. The Fairfield Four, Traditional, arr. by the Fairfield Four - Lonesome Valley | not rated | 4:07 |
| 19. The Stanley Brothers, Traditional, arr. by Ralph Stanley - Angel Band | not rated | 2:16 |
| 1:00:35 |
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