| 1. James Carter & The Prisoners, James Carter & The Prisoners - Po Lazuras | not rated | 4:31 |
| 2. Harry McClintock, Harry Kirby McClintock - Big Rock Candy Mountain | not rated | 2:17 |
| 3. Norman Blake, Jimmie Davies/Charles Mitchell - You Are My Sunshine | not rated | 4:27 |
| 4. Alison Krauss, (Traditional) - Down To The River To Pray | not rated | 2:55 |
| 5. Soggy Bottom Boys feat. Dan Tyminski, (Traditional) - I Am A Man Of Constant Sorrow <Radio Station Version> | not rated | 3:10 |
| 6. Chris Thomas King, Skip James - Hard Time Killing Floor Blues | not rated | 2:42 |
| 7. Norman Blake, (Traditional) - I Am A Man Of Constant Sorrow <Instrumental> | not rated | 4:29 |
| 8. The Whites, A P Carter - Keep On The Sunny Side | not rated | 3:34 |
| 9. Gillian Welch & Alison Krauss, Albert E Brumley - I'll Fly Away | not rated | 3:57 |
| 10. Gillian Welch, Alison Krauss & Emmylou Harris, (Traditional) - Didn't Leave Nobody But The Baby | not rated | 1:57 |
| 11. The Peasall Sisters, Maybelle Carter - In the Highways | not rated | 1:36 |
| 12. The Cox Family, Pete Roberts - I Am Weary (Let Me Rest) | not rated | 3:14 |
| 13. John Hartford, (Traditional) - I Am A Man Of Constant Sorrow <Instrumental> | not rated | 2:34 |
| 14. Ralph Stanley, (Traditional) - O Death | not rated | 3:19 |
| 15. Soggy Bottom Boys feat. Tim Blake Nelson, Jimmie Rodgers - In The Jailhouse Now | not rated | 3:36 |
| 16. Soggy Bottom Boys feat. Dan Tyminski, (Traditional) - I Am A Man Of Constant Sorrow <with Band> | not rated | 4:16 |
| 17. John Hartford, Hoyt Ming - Indian War Whoop | not rated | 1:30 |
| 18. The Fairfield Four, (Traditional) - Lonesome Valley | not rated | 4:07 |
| 19. The Stanley Brothers, (Traditional) - Angel Band | not rated | 2:15 |
| 1:00:35 |
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